Unveiling novel scallop-derived antimicrobial peptides targeting host-associated vibrios for sustainable pathogen control in aquaculture

• Published in: Aquaculture Vol. 592; p. 741238
• Main Authors: Revilla, Jormil, Stambuk, Felipe, Hurtado, Luz, Rojas, Rodrigo, Aróstica, Mónica, Guzmán, Fanny, Cárdenas, Constanza, Álvarez, Claudio A., Brokordt, Katherina, Schmitt, Paulina
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.11.2024
• Subjects: Antimicrobial peptides; Host-derived antimicrobials; Scallop aquaculture; Sustainable aquaculture; Vibrios; Sustainable aquaculture; Host-derived antimicrobials; Scallop aquaculture; Vibrios; Antimicrobial peptides
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2024.741238

Scallop aquaculture is threatened by Vibrio bacteria-induced larval mortality, needing the development of sustainable pathogen control strategies. This study investigates the potential of host-derived antimicrobial peptides (AMPs) as eco-friendly alternatives for pathogen control in aquaculture. Exploiting the scallop transcriptome, we identified sequences sharing common AMP features and designed a de novo peptide named helixidin, a 40-amino acid peptide with a net charge of +11 and a pI of 11.52. In addition, we designed an analog, N-ter ApBD Q13R, based on the hydrophobic domain of the scallop big defensin ApBD, with a glutamine-to-arginine substitution at position 13. N-ter ApBD Q13R is a 35-residue peptide with a net charge of +4 and a pI of 11.55. The synthetic peptides helixidin and N-ter ApBD Q13R adopted an alpha-helix secondary structure with an amphiphilic distribution of amino acid residues. Circular dichroism assays confirmed their structural stability across varying pH, osmolarity, and temperature conditions. Moreover, these peptides displayed potent broad-spectrum antibacterial activity, in the case of helixidin causing cell wall and membrane disruption of Gram-positive and Gram-negative bacteria, respectively. On the other hand, N-ter ApBD Q13R showed a possible penetrating action on bacterial cells, with formation of nanonets-like structures against the scallop pathogen Vibrio bivalvicida VPAP30. Notably, both peptides exhibited selective activities against Vibrio strains isolated from healthy scallop larvae and specific bivalve pathogenic Vibrio, underscoring their potential for targeting host-associated vibrios. Biosafety tests showed that both peptides are harmless to A. purpuratus larvae. In conclusion, helixidin and N-ter ApBD Q13R represent novel cationic AMPs with different mechanisms of action against host-associated vibrios, offering promising prospects for sustainable pathogen control in scallop aquaculture. •Two novel AMP derived from the scallop A. purpuratus transcriptome were designed.•Synthetic helixidin and N-ter ApBD Q13R are alpha helical peptides with broad thermal stability.•Both synthetic peptides showed selected antibacterial activity against pathogenic Vibrio strains.•Helixidin destroys the wall and membrane of bacteria, while N-ter ApBD Q13R possibly has penetrating action.•Both synthetic peptides are innocuous for larvae of A. purpuratus.