Expression and purification of Artogeia rapae lysozyme Ⅱ cDNA in a baculovirus/insect cell system

Previously, the Artogeia rapae lysozyme Ⅱ (ARLⅡ) gene was isolated and its complete nucleotide sequence was determined by RACE-PCR from fat body of larvae injected with Escherichia coli. In the present study, the ARLⅡ gene was expressed by using a baculovirus expression vector system (BEVS). The exp...

Full description

Saved in:
Bibliographic Details
Published inEntomological research Vol. 36; no. 2; pp. 116 - 121
Main Authors Bang, I.S. (MyGene Bioscience Institute, Seoul, Republic of Korea), Yoe, S.M. (Dankook University, Cheonan, Republic of Korea), Kang, C.S. (Hoseo University, Asan, Republic of Korea), E-mail: cskang@office.hoseo.ac.kr
Format Journal Article
LanguageEnglish
Published Melbourne, Australia Blackwell Publishing Asia 01.06.2006
한국곤충학회
Subjects
Artogeia rapae
BACULOVIRIDAE
baculovirus
c-type lysozyme
insect immunity
생물학
Online AccessGet full text

Cover

More Information
Summary:Previously, the Artogeia rapae lysozyme Ⅱ (ARLⅡ) gene was isolated and its complete nucleotide sequence was determined by RACE-PCR from fat body of larvae injected with Escherichia coli. In the present study, the ARLⅡ gene was expressed by using a baculovirus expression vector system (BEVS). The expression level of recombinant (r)ARLⅡ protein was optimized by varying virus titer and time-course of infection. The optimum protein expression conditions were infection of the cells at a multiplicity of infection of 10, and harvest at 84 h post-infection.
Bibliography:2007001919
H10
istex:2DEE4790D884CCEDDDA367AD9D062392089BDF0F
ArticleID:ENR019
ark:/67375/WNG-82HX25R2-2
G704-000624.2006.36.2.006
ISSN:1738-2297
1748-5967
1748-5967
DOI:10.1111/j.1748-5967.2006.00019.x