IN VIVO EFFECT OF Origanum vulgare ESSENTIAL OIL ON MICROBIOLOGICAL QUALITY OF RAW MILK FROM SUBCLINICAL MASTITIS AFFECTED COWS

The tested oil extracted from Algerian endemic oregano collected in Setif province (eastern Algeria) was characterised as carvacrol-chemotype using gas chromatography-mass spectrometry (GC-MS). [...]the use of essential oils as a possible alternative therapeutic approach against mastitis to solve th...

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Published inPoljoprivreda i šumarstvo Vol. 67; no. 3; pp. 83 - 98
Main Authors Walid HAMLAOUI, Mohamed, HADEF, Ahmed, Rostane MEKLATI, Fawzi, ALLEG, Meriem, GHOUILA, Bouchra
Format Journal Article
LanguageEnglish
Published Titograd University of Montenegro, Biotechnical Faculty 30.09.2021
Subjects
Antibiotic resistance
Antibiotics
Antimicrobial agents
Bacteria
Biomedical materials
Carrier gases
Carvacrol
Chemical composition
Chromatography
Clinical trials
Dairy farms
Essential oils
Flow rates
Flow velocity
Gas chromatography
Helium
In vivo methods and tests
Livestock
Mass spectrometry
Mass spectroscopy
Mastitis
Milk
Oils & fats
Oregano
Scientific imaging
Spectroscopy
Vegetable oils
United States > US
Algeria
California
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Summary:The tested oil extracted from Algerian endemic oregano collected in Setif province (eastern Algeria) was characterised as carvacrol-chemotype using gas chromatography-mass spectrometry (GC-MS). [...]the use of essential oils as a possible alternative therapeutic approach against mastitis to solve the problem of antibiotic resistance has been the subject of several in vitro experiments, but their properties have been very little tested in vivo on livestock (Baskaran et al., 2009; Mushtaq et al., 2018; Alagawany et al., 2020; Gupta et al., 2020). Characterisation of oregano essential oil used in the clinical trial The chemical composition of OEO to be used in the in vivo test was identified by gas chromatography-mass spectrometry (GC-MS). GC-MS spectra were obtained using the following conditions: injected essential oil volume was 1 pl in splitless mode; injection temperature was 250 °C; helium was the carrier gas at a flow rate of 0.5 ml/min; oven temperature program (60 °C for 8 min, increased at a rate of 2 °C/min to 250 °C, then held at 250 °C for 10 min).
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ISSN:0554-5579
1800-9492
DOI:10.17707/AgricultForest.67.3.07