Screening of xylanase activity of Streptomyces albidoflavus PSM-3n isolated from Uttarakhand

• Published in: Eurasian journal of biosciences Vol. 2013; no. 7; pp. 30 - 40
• Main Authors: Sharma, Pushpendra, Kumar, Vijay, Naik, Bindu, Bisht, Gajraj Singh
• Format: Journal Article
• Language: English
• Published: EurAsian Journal of BioSciences 2013
• Subjects: Coğrafya; İzmir; Türkiye; soil; xylanase activity; Agro-industrial end products; Streptomyces sp
• ISSN: 1307-9867; 1307-9867
• DOI: 10.5053/ejobios.2013.7.0.4

Background: Awareness towards the environmental pollution had made the evolution of greentechnology by which enzymes got special attention in industries. The enzymes replaced chemicalcatalysts in manufacturing various chemicals, agricultural and pharmaceutical products.Material and Methods: Actinomycetes were isolated and screened for their ability to producexylanase. For the most promising isolate, selection of media, effect of pH, temperature, metal ions,and detergents on enzyme production and activity was studied.Results: Out of 29 isolates, 22 isolates showed xylanase activity. Out of 22 xylanase producingisolate, 05 isolates were selected for secondary screening on the basis of their clear zone size. Themost promising isolate PSM-3n was identified as Streptomyces albidoflavus. It produces maximumenzyme (xylanase) in media Horikoshi and Ikura having carbon and nitrogen sources as oat mealand urea respectively. The optimum pH and temperature for the enzyme production was 4.0 and45ºC respectively. The enzyme activity was found maximum at temperature 50ºC and enhanced inthe presence of Fe3+ ions. There was a reduction in the enzyme activity in the presence of detergentslike SDS, tween-20 and tween-80. The enzyme was fairly stable at 50°C for 1 h.Conclusions: The enzyme produced by the isolate PSM-3n is fairly heat stable and highly acidstable. The activity of the enzyme was increased in presence of Fe3+ ions while decreased inpresence of SDS. Therefore, further studies are required for purification of xylanase for itsapplication potential in pulp bioleaching processes and in the functional food industry.