Mitochondrial respiration of the rat liver in biliary obstruction

• Published in: Kanzo Vol. 20; no. 6; pp. 579 - 586
• Main Authors: KOYAMA, Kenji, ITO, Kenji, OTOWA, Tsuyoshi, YAMAUCHI, Hidemi, TAKAGI, Yasushi, OWADA, Yasuo, OUCHI, Kiyoaki, WATABE, Shuichi, MATSUBARA, Shuji, MITSUI, Teruo, SATO, Toshio
• Format: Journal Article
• Language: Japanese
• Published: The Japan Society of Hepatology 1979
• ISSN: 0451-4203; 1881-3593
• DOI: 10.2957/kanzo.20.579

Mitochondrial function of the rat liver in biliary obstruction was studied. Respiratory parameters of mitochondria such as RC, P/O, S3, ATP synthesis, and mitochondrial cytochrome a, c were reduced with the prolongation of bile duct obstruction. Latent ATPase activity was elevated in early stage of jaundice but decreased in long term obstruction. DNP stimulated ATPase showed same changes as latent ATPase and the ratio of latent ATPase to DNP stimulated ATPase was increased with the prolongation of biliary obstruction, which indicated the deterioration of injury of mitochondrial membrane. The role of bile acids as one of the respiratory inhibitors was examined in vitro; free CDCA inhibited most remarkably, conjugated CDCA did next and both free and conjugated CA did less. In hypotension induced in jaundiced rats by exanguination, mitochondrial respiration was inhibited more prominently than that without hypotension.