Antibiogram of the Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus epidermidis and Staphylococcus intermedius isolated from the bovine frozen semen

• Published in: Pure and applied biology Vol. 5; no. 2; pp. 204 - 212
• Main Authors: Abro, Shahid Hussain, Abro, Rani, Rind, Rahamatullah, Kamboh, Asghar Ali, Memon, Akeel Ahmed, Channa, Aijaz Ali, Wagan, Hakimzadi, Baloch, Hassina, Wagan, Bakhtawar
• Format: Journal Article
• Language: English
• Published: Quetta International Society of Pure and Applied Biology 01.06.2016
• Subjects: Animals; Antibiotics; Artificial insemination; Bacteria; Cattle; Drug resistance; Fermentation; Laboratories; Micrococcus luteus; Pseudomonas aeruginosa; Quality; Staphylococcus epidermidis; Staphylococcus intermedius
• ISSN: 2304-2478; 2304-2478
• DOI: 10.19045/bspab.2016.50027

The presence of harmful microbes may negatively influence semen quality. Antibiotic used in the semen extender should be evaluated in order to prevent contamination and infertility. In this study, Antibiogram evaluation of Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus epidermidis and Staphylococcus intermedius were detected from frozen semen of cattle. Different antibiotics such as amikacin, ampicillin, amoxicillin, cephalexin, erythromycin, gentamicin, neomycin, ofloxacin and sulphamethoxazole/trimethoprim were tested against the isolated bacterial species. Ofloxacin, amikacin, cephalexin and amoxicillin were highly effective against Micrococcus luteus. The organism was found equally effective to erythromycin, gentamycin and neomycin. Amikacin, ofloxacin, erythromycin, neomycin and ampcillin were the most effective against the Pseudomonas aeruginosa. However, amoxicillin, cephalexin and sulphamethoxazole/trimethoprim were not shown any response. Staphylococcus epidermidis was found highly sensitive to amikacin. While antibiotics amoxicillin, erythromycin, and cephalexin completely failed to give response against the organism. Staphylococcus intermedius was noted highly sensitive to neomycin, amikacin and ampicillin. Overall, amikacin and neomycin were found the most effective antibiotics against these bacterial isolates determined from bovine semen samples.