Evaluation of in vitro Paper-strip Uptake of Liquid Containing Chondroitin 4-sulfate

• Published in: Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Vol. 44; no. 2; pp. 117 - 130
• Main Authors: Nagasawa, Nobutake, Kanazawa, Atsushi, Iwayama, Yukio
• Format: Journal Article
• Language: Japanese
• Published: JAPANESE SOCIETY OF PERIODONTOLOGY 2002
• Subjects: chondroitin 4-sulfate; in vitro; strips
• ISSN: 0385-0110; 1880-408X
• DOI: 10.2329/perio.44.117

Sulfated glycosaminoglycan in gingival crevicular fluid is favored as a biomarker of possible boneresorption. Despite common use of filter paper to collect gingival crevicular fluid, little attention has been paid to the role of the type of filter paper, despite it being a critical factor in determining fluid uptake and migration. We assessed in vitro paper suitability and its subsequent quantification of sulfated glycosaminoglycans. Samples of fluid containing chondroitin 4-sulfate wcrc collected on diffcrcnt paper strips. After sampling, strips were stained directly with 0.2% alcian blue solution containing 0.05 M MgCl2, 0.4 M guanidine-HCl, 0.02 M sulfuric acid, and 0.25% Triton X-100 at pH 1.5, and analyzed them using a chromatoscanner. The cellulose acetate membrane (Separax (R)) strip was found to be the most suitable for recovering chondroitin 4-sulfate in fluid. It was superior in staining of chondroitin 4-sulfate, fluid adsorption, and background destaining. Our in vitro study suggests that sampling time was less than 60 seconds and fluid volume collected by Separax (R) paper strips was 3 microliters. J Jpn Soc Periodontol, 44: 117-130, 2002.