Spontaneous cell-mediated cytotpxicity to cultured rat liver cells mediated by normal peripheral blood lymphocytes

• Published in: Kanzo Vol. 20; no. 8; pp. 773 - 781
• Main Authors: NONOMURA, Akitaka, HARATAKE, Joji, KARINO, Tetsuji, OHTA, Goroku
• Format: Journal Article
• Language: Japanese
• Published: The Japan Society of Hepatology 1979
• Subjects: ADCC; NKcell; SCMC
• ISSN: 0451-4203; 1881-3593
• DOI: 10.2957/kanzo.20.773

Spontaneous cell-mediated cytotoxicity (SCMC) against cultured rat liver cells (Coon cells) was investigated in healthy peripheral blood Iymphocytes using microcytotoxicity assay. Cytotoxic activity was found in fraction of EA-RFC, EAC-RFC, low affinity ERFC, aminoethylisothiuronium bromide (AET)-treated E-RFC and weak adherent cell showing % cytotoxicity of 40.0±6.3 (Mean±SD), 38.3±7.0, 21.8±16.2, 20.6±14.3 and 35.1±10.9, respectively. These results indicated heterogeneity in subpopulation of the cytotoxic lymphocytes mediating SCMC. Analysis of lymphocyte subsetS adherent to target Coon cells after 15 hrs incubation revealed that EA-RFC, EAC-RFC and E-RFC were 50.2±1.8%, 50.9±9.1% and 12.6±4.7%, respectively. Some E, EA and EAC were found adherent to target Coon cells showing cytopathological alterations which appears attributable to the lymphocyte cytotoxicity, whereas, only few E, EA and EAC, if present, were adherent to normal Coon cells. It would appear, therefore, that receptor-like structures to E, EA and EAC might be formed on the surface of damaged target cells during incubation time with cytotoxic lymphocytes. Also, it is likely that changes of the membrane charge due to cytopathological alterations may cause non-specific bindings. And possible significance of SCMC in hepatocyte injury was discussed.