Effect of Culture Media on Seed Dormancy and Callus Induction Ability of Some Wild and Cultivated Rice Genotypes

Seed dormancy status was evaluated using agar, MS media and DKW media with or without sugar (3%) in two wild (Jhora 4325 and Wild 4855) and three cultivated (Hab. Aman 2, Jagliboro and Dular) rice genotypes. Jhora 4325 showed strong seed dormancy than the wild 4855 and cultivated rice genotypes. Emb...

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Published inBiotechnology (Faisalābād, Pakistan) Vol. 6; no. 1; pp. 61 - 63
Main Authors Hoque, M N, Rahman, L, Hassan, L
Format Journal Article
LanguageEnglish
Published 2007
Subjects
Freshwater
Oryza sativa
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Summary:Seed dormancy status was evaluated using agar, MS media and DKW media with or without sugar (3%) in two wild (Jhora 4325 and Wild 4855) and three cultivated (Hab. Aman 2, Jagliboro and Dular) rice genotypes. Jhora 4325 showed strong seed dormancy than the wild 4855 and cultivated rice genotypes. Embryo germination percentage in the studied rice genotypes was higher in agar and MS media than DKW media. Number and percent explants with Induced callus was comparatively higher in MS media in combination with 2, 4-D (1.5 mg L super(-1) 2, 4-D) and BAP (0.5 mg L super(-1)) than MS media supplemented with 2, 4-D alone. Embryogenic callus initiation capability of wild rice genotypes was higher than the cultivated genotypes. Embryo germination and callus induction were influenced by concentration of plant growth regulators, culture media and rice genotypes.
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ISSN:1682-296X
DOI:10.3923/biotech.2007.61.63