Behavior of Two and Three Electrode Configuration and Different Mediators in Working Electrode on Development of Disposable Screen-Printing Biosensors for Determination of Free Cholesterol

In the present work the development of an amperometric transducer in order to build a free cholesterol biosensor in planar configuration is reported. The one single use disposable biosensors were constructed by screen printing process which is compatible with automated methodologies of production. B...

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Bibliographic Details
Published inJournal of the Mexican Chemical Society Vol. 57; no. 1
Main Authors Hernández-Cruz, Mónica, Galán-Vidal, Carlos Andrés, Álvarez-Romero, Giaan Arturo, Ramírez-Silva, María Teresa, Páez-Hernández, María Elena, González-Vidal, José Luis
Format Journal Article
LanguageEnglish
Published 12.10.2017
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Summary:In the present work the development of an amperometric transducer in order to build a free cholesterol biosensor in planar configuration is reported. The one single use disposable biosensors were constructed by screen printing process which is compatible with automated methodologies of production. Based in the incorporation of tetracyanoquinodimethane (TCNQ), Prussian blue (PB) or ferrite (Fe3O4) as mediator or electrocatalytic agent, three types of electrochemical transducers were evaluated. The cholesterol biosensors require a sample volume of 7.2 μL, exhibits good reproducibility and selectivity and cover a lineal answer of 2-16 mM with detection limits from 0.3 to 1.6 mM. The characteristics of biosensors are satisfactory for the decentralized analysis of the lipid in blood since enclose the range of clinical interest (3.5-6.5 mM).
ISSN:1870-249X
2594-0317
DOI:10.29356/jmcs.v57i1.237