tra region of the natural conjugative Escherichia coli plasmid pRKlOO is F-like

The aim of the presented study was to identify the similarity of the pRKI00 tra region with tra regions of other conjugative plasmids of Enterobacteriaceae.For this purpose several tra genes were amplified with PCR and the nucleotide sequences of the obtained PCR products were determined. The pRKI O...

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Bibliographic Details
Published inActa Biologica Slovenica Vol. 45; no. 1
Main Authors Marjanca Starčič Erjavec, Wim Gaastra, Darja Žgur-Bertok
Format Journal Article
LanguageEnglish
Published University of Ljubljana Press (Založba Univerze v Ljubljani) 01.07.2002
Subjects
conjugative plasmid
Enterobacteriaceae
tra region
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Summary:The aim of the presented study was to identify the similarity of the pRKI00 tra region with tra regions of other conjugative plasmids of Enterobacteriaceae.For this purpose several tra genes were amplified with PCR and the nucleotide sequences of the obtained PCR products were determined. The pRKI OO's nucleotide sequences were compared to the nucleotide sequences deposited in GenBank and the nucleotide divergence between them was calculated. The obtained results clearly demonstrated, that the tra region of pRKI0O is the most similar to the tra region of plasmid F.
ISSN:1854-3073
DOI:10.14720/abs.45.1.16624