Effect of matrine on expression of HCCR1 and HCCR2 proteins in cultural human hepatocellular carcinomas cells

R735.7; Objective: To explore the effects of matrine on HCCR1 and HCCR2 expression in cultural human hepatocellular carcinomas (HCC) cells at the level of gene and protein. Methods: Three methods, representational difference analysis(RDA) of cDNA, microarrays and fluorescence in situ hybridization (...

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Bibliographic Details
Published inChinese journal of cancer research Vol. 17; no. 4; pp. 265 - 269
Main Authors Geng, Dong-jin, Chen, Jun-hao, Chen, Lei-lei, Gu, Xiang-fang, Zhang, Le, Han, Li, Liu, Yong
Format Journal Article
LanguageEnglish
Published Molecular Medicine Center and Medical Laboratory Center, Affiliated Drum Tower Hospital,Nanjing University, Nanjing 210008 01.12.2005
Subjects
Human HCCR1 and HCCR2 genes
Matrine
Hepatocellular carcinomas cells
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Summary:R735.7; Objective: To explore the effects of matrine on HCCR1 and HCCR2 expression in cultural human hepatocellular carcinomas (HCC) cells at the level of gene and protein. Methods: Three methods, representational difference analysis(RDA) of cDNA, microarrays and fluorescence in situ hybridization (FISH) were used to detect levels of mRNA and protein expression of HCCR1 and HCCR-2 before and after treatment of matrine. Results: Matrine had inhibitory effect on the mRNA and protein expression of HCCR1 and HCCR2 in cultural HCC cells. Conclusion: Matrine has inhibitory effect on gene transcription, protein expression of HCCR1 and HCCR2 in cultural HCC cells.
ISSN:1000-9604
1993-0631
DOI:10.1007/s11670-005-0023-4