Isolation and Functional Characterization of the Chick M 5 Muscarinic Acetylcholine Receptor Gene

The chick is a widely used system for study of the actions of muscarinic acetylcholine receptors in the cardiovascular, visual, and nervous systems. We report the isolation and functional analysis of the gene encoding the chick M 5 muscarinic receptor. RT‐PCR analysis indicates that the M 5 receptor...

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Bibliographic Details
Published inJournal of neurochemistry Vol. 74; no. 2; pp. 882 - 885
Main Authors Creason, Sharon, Tietje, Kathleen M., Nathanson, Neil M.
Format Journal Article
LanguageEnglish
Published 01.02.2000
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Summary:The chick is a widely used system for study of the actions of muscarinic acetylcholine receptors in the cardiovascular, visual, and nervous systems. We report the isolation and functional analysis of the gene encoding the chick M 5 muscarinic receptor. RT‐PCR analysis indicates that the M 5 receptor is expressed at low levels in embryonic chick brain and heart. When expressed in stably transfected Chinese hamster ovary cells, the M 5 receptor exhibits high‐affinity binding to muscarinic antagonists and mediates robust activation of phospholipase C activity.
ISSN:0022-3042
1471-4159
DOI:10.1046/j.1471-4159.2000.740882.x