Assessing lipid nanoparticle protein corona formation and cytocompatibility

Lipid nanoparticles (LNPs) represent an emerging new modality for mRNA delivery. Following administration and interaction with blood constituents, LNPs form a corona complex consisting of proteins adsorbed on the surface altering their stability, biological identity, and fate. Cytocompatibility of t...

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Published inBritish Journal of Pharmacy Vol. 8; no. 2; pp. S1 - S2
Main Authors Abdulrahman, Rand, Ibrahem, Huda, Mahmood, Amna, Treacher, Kevin Edward, Capomaccio, Robin Bruno, Vasey, Catherine, Perrie, Yvonne, Rattray, Zahra
Format Journal Article
LanguageEnglish
Published Huddersfield, United Kingdom University of Huddersfield Press 01.12.2023
Subjects
analysis
Cells
cytocompatibility
Light
lipid nanoparticles
Measurement
Membrane lipids
Metabolism
Metal nanoparticles
protein corona
Regulation
Scattering
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Summary:Lipid nanoparticles (LNPs) represent an emerging new modality for mRNA delivery. Following administration and interaction with blood constituents, LNPs form a corona complex consisting of proteins adsorbed on the surface altering their stability, biological identity, and fate. Cytocompatibility of the LNPs is an important factor when considering their safety efficacy in delivering the encapsulated drug dose, lipid choice and the specific target cells. The aim of this study was to investigate the changes in LNP physical parameters in physiologically-relevant media. Key attributes such as particle size, polydispersity index and zeta-potential were measured using Dynamic Light Scattering (DLS) and Nanoparticle Tracking Analysis (NTA). Cytocompatibility was assessed via CellTiter-Glo assay. Following 24-hour inCubation of LNPs with Bovine Serum Albumin (BSA), the LNP z-average increased from 92.4 (+- 49.0) nm to 131.4 (+- 64.9) nm indicating interaction between LNPs and BSA. A decrease in percentage cell viability was demonstrated with increased lipid concentration for MCF-7 and A549 cell lines. This work shows changes in LNP physicochemical properties in the presence of protein and biologically relevant conditions consistent with protein surface adsorption. The cytocompatibility of LNPs can be associated with the type of lipids used in the synthesis of LNPs.
Bibliography:British Journal of Pharmacy, Vol. 8, No. 2, Dec 2023, S1-S2
Informit, Melbourne (Vic)
ISSN:2058-8356
2058-8356
DOI:10.5920/bjpharm.1398