DNA Mediated Sequential Self-assembly of Au Nano-particles

The novel sequential self-assembly process applying hybridization of DNA mediated micro/nano components was proposed and its validity was experimentally verified. In the proposed process, characteristic of DNA, such as hybridization specificity of complementary pairs of single-stranded DNA (ssDNA) i...

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Bibliographic Details
Published inFuntai Kogakkaishi Vol. 45; no. 3; pp. 156 - 161
Main Authors Kusakabe, Tatsuya, Tanemura, Tomoki, Higuchi, Yuichi, Sugano, Koji, Tsuchiya, Toshiyuki, Tabata, Osamu
Format Journal Article
LanguageEnglish
Published The Society of Powder Technology, Japan 2008
Subjects
DNA
Gold nano-particle
Hybridization
Self-assembly
Sequential
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Summary:The novel sequential self-assembly process applying hybridization of DNA mediated micro/nano components was proposed and its validity was experimentally verified. In the proposed process, characteristic of DNA, such as hybridization specificity of complementary pairs of single-stranded DNA (ssDNA) into a double-stranded DNA (dsDNA), and its dependence on melting temperature Tm, which can be designed by the base-pair sequence and salt-concentration of surrounding media, was utilized to control the self-assembly sequence of a micro/nano components modified by ssDNA. Au nanoparticles with 15nm diameter were used as components and two pairs complementary DNA with different Tm were prepared. The aggregation of Au nano-particles modified by complementary ssDNA was confirmed at different temperatures with decreasing temperature under Tm. The reversibility of this process was also confirmed by increasing temperature.
ISSN:0386-6157
1883-7239
DOI:10.4164/sptj.45.156