Influence of Water Activity on the Amount of Adenosine Triphosphate (ATP) of Bacteria by Bioluminescence

ATP levels of bacteria in Tryptic Soy Broth with 0.5 to 8.7% (w/w) NaCl (water activity, aw 0.997-0.945) were studied during 3-day incubation period at 25°C by the luciferin-luciferase bioluminescence reaction method. The amount of intracellular ATP per cell of Staphylococcus epidermidis, Escherichi...

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Bibliographic Details
Published inJapanese Journal of Food Microbiology Vol. 16; no. 3; pp. 201 - 204
Main Authors ONOUE, Youichi, TATSUGUCHI, Hisako, FURUKAWA, Ichiro, TERANISHI, Hiroshi, HASEGAWA, Yukie
Format Journal Article
LanguageEnglish
Published Japanese Society of Food Microbiology 1999
Subjects
ATP
Bacteria
NaCl
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Summary:ATP levels of bacteria in Tryptic Soy Broth with 0.5 to 8.7% (w/w) NaCl (water activity, aw 0.997-0.945) were studied during 3-day incubation period at 25°C by the luciferin-luciferase bioluminescence reaction method. The amount of intracellular ATP per cell of Staphylococcus epidermidis, Escherichia coli and Vibrio parahaemolyticus ranged from 1.3 to 6.1×10-18 mol/cell in the stationary phase. ATP content of bacterial cells varied greatly with age. The changes in aw had little effect upon the amount of ATP.
ISSN:1340-8267
1882-5982
DOI:10.5803/jsfm.16.201