Cross-linking of initiation factor IF3 to Escherichia coli 30S ribosomal subunit by trans-dlamminedichloroplatinum(II): characterization of two cross-linking sites in 16S rRNA; a possible way of functioning for IF3

SUMMARY The Initiation factor IF3 is platinated with trans-d1anm1ne-d1chloroplat1num(II) and cross-linked to Escherichia coli 30S ribosomal subunit. Two cross-linking sites are unambiguously identified on the 16S rRNA : a major one, in the region 819–859 1n the central domain, and a minor one, 1n th...

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Published inNucleic acids research Vol. 14; no. 12; pp. 4803 - 4821
Main Authors Ehresmann, Chantal, Moine, Hervè, Mougel, Marylène, Dondon, Jacques, Grunberg-Manago, Marianne, Ebel, Jean-Pierre, Ehresmann, Bernard
Format Journal Article
LanguageEnglish
Published Oxford University Press 25.06.1986
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Summary:SUMMARY The Initiation factor IF3 is platinated with trans-d1anm1ne-d1chloroplat1num(II) and cross-linked to Escherichia coli 30S ribosomal subunit. Two cross-linking sites are unambiguously identified on the 16S rRNA : a major one, in the region 819–859 1n the central domain, and a minor one, 1n the region 1506–1529 1n the 3'-term1nal domain. Specific features of these sequences together with their particular location within the 30S subunit lead us to postulate a role for IF3, that conciliates topographical and functional observations made so far.
Bibliography:ark:/67375/HXZ-85MB6HL2-P
istex:9FE369B950AB66C4C933BF0F05B209BB5F746AEC
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ArticleID:14.12.4803
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/14.12.4803