Flavonoids from aerial part of Algerian Ajuga iva (L.) schreb.: The HPLC-UV analysis and Antioxidant capacity

• Published in: Kragujevac Journal of Science Vol. 2021; no. 43; pp. 23 - 34
• Main Authors: Boukada, Fadhela, Meddah, Boumediene
• Format: Journal Article
• Language: English
• Published: University of Kragujevac, Faculty of Science 2021
• Subjects: antioxidant effect; flavonoids; hplc; polyphenols
• ISSN: 1450-9636; 2466-5509
• DOI: 10.5937/KgJSci2143023B

The study deals with the evaluation of the antioxidant capacity of extracts from the aerial part of Algerian Ajuga iva. Extraction of flavonoids was carried out by 85% of methanol, then the crude extract was successively separated with ethyl acetate, butanol, and water. The in vitro antioxidant activity was assessed by 1,1-diphenyl-2-picrylhydrazyl, reducing power, and thiobarbituric acid reacting substances assays. Extracts are subject to HPLC-UV analysis. The average total phenol contents of extracts vary between 3.87 ± 0.17 and 149.74 ± 3.94 (gallic acid equivalent per gram of dry extract). Furthermore, tested extracts exhibited a broad range of flavonoid contents varying from 1.54 ± 0.09 to 41.18 ± 1.03 (catechin equivalent per gram of dry extract). Butanol and ethyl acetate fractions displayed the highest antioxidant activity. A good correlation between the phenolic and flavonoid contents and the antioxidant activity was observed. Rutin, caffeic acid, quercetin, p-coumaric acid, luteolin, and cinnamic acid were present in the extracts. The plant could be a potential source of antioxidant agents.