Biocompatability chitosan /Aloe barbadensis miller loaded moxifloxacin composite film: A multifunctional bio-platform for the treatment of breast cancer

• Published in: Journal of molecular structure Vol. 1315; p. 138618
• Main Authors: Rengasamy, Gowri, Mahalingam, Sundrarajan, Udaiyar, Thamayanthi, Kalirajan, Meenatchi
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 05.11.2024
• Subjects: Aloe barbadensis miller; Chitosan; Cytotoxicity; Hydrophilic; MCF-7; Moxifloxacin; Aloe barbadensis miller; Cytotoxicity; Hydrophilic; Chitosan; Moxifloxacin; MCF-7
• ISSN: 0022-2860
• DOI: 10.1016/j.molstruc.2024.138618

•The CS-ABM/MoF composite film was fabricated using a solution casting method.•The plasticizer of ABM improves the film's physicochemical properties.•Improved hydrophilicity to induce swelling ratio for biodegradability.•Enhance drug release (80 %) at 24 h for sustainable and control killing efficiency to cancer cells.•Zone of inhibition of S.aureus (ATCC33591) 21 ± 1.0 mm and E.coli (ATCC10536) 22 ± 1.4 mm. Binary biodegradable polymers exhibit excellent functional behavior but the limited mechanical integrity for regulating drug release within the polymeric matrix, hence it needs modification in the polymer network to overcome the limitations. The compatibility of bipolymer blends with incorporation of drugs within their matrices to enhance physicochemical properties and drug delivery kinetics have garnered significant attention in recent research. Herein, we examine the influence of the film produced by the casting technique, which consists of chitosan (CS) and Aloe barbadensis miller (ABM) gel, embedded with moxifloxacin (MoF), on attenuating the breast cancer (MCF-7) cell line. Firstly, spectroscopy confirmed the intermolecular interactions between the matrixes and the grafting of components. Notably, the addition of ABM produces significant changes in the film properties, such as decreased elongation. The pivotal moment resulted in the generation of more stable films that displayed enhanced stability at temperatures below 200 °C, as confirmed by TGA analysis. Additionally, film surfaces exhibiting strong water affinity were found to be more favorable for cell adhesion, subsequently hindering the proliferation of compromised cells. Remarkably, the In vitro cytotoxicity study showed the antibiotic-grafted CS-ABM/MoF scaffolds exhibited high cell adhesion and improved the rate of cell death was higher compared to other films. The antibacterial activity against S. aureus and E. coli results strongly suggests the enhanced antibiotic ability of the polymeric CS- ABM/MoF film. These results findings indicated that the CS- ABM/MoF scaffolds' hydrophilic surface significantly contributes to the improved cell adhesion efficiently used for biomedical applications. [Display omitted]