Community-integrated multi-omics facilitates the isolation of an organohalide dehalogenation microorganism

• Published in: Innovation (New York, NY) Vol. 4; no. 1; p. 100355
• Main Authors: Huang, Yiqun, Wen, Lingyu, Zhang, Lige, Xu, Jijun, Wang, Weiwei, Hu, Haiyang, Xu, Ping, Li, Zhao, Tang, Hongzhi
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 30.01.2023
• Subjects: metagenome assembled genome; microbial community; organohalide; wastewater; wastewater; microbial community; organohalide; metagenome assembled genome
• ISSN: 2666-6758; 2666-6758
• DOI: 10.1016/j.xinn.2022.100355

A variety of anthropogenic organohalide contaminants generated from industry are released into the environment and thus cause serious pollution that endangers human health. In the present study, we investigated the microbial community composition of industrial saponification wastewater using 16S rRNA sequencing, providing genomic insights of potential organohalide dehalogenation bacteria (OHDBs) by metagenomic sequencing. We also explored yet-to-culture OHDBs involved in the microbial community. Microbial diversity analysis reveals that Proteobacteria and Patescibacteria phyla dominate microbiome abundance of the wastewater. In addition, a total of six bacterial groups (Rhizobiales, Rhodobacteraceae, Rhodospirillales, Flavobacteriales, Micrococcales, and Saccharimonadales) were found as biomarkers in the key organohalide removal module. Ninety-four metagenome-assembled genomes were reconstructed from the microbial community, and 105 hydrolytic dehalogenase genes within 42 metagenome-assembled genomes were identified, suggesting that the potential for organohalide hydrolytic dehalogenation is present in the microbial community. Subsequently, we characterized the organohalide dehalogenation of an isolated OHDB, Microbacterium sp. J1-1, which shows the dehalogenation activities of chloropropanol, dichloropropanol, and epichlorohydrin. This study provides a community-integrated multi-omics approach to gain functional OHDBs for industrial organohalide dehalogenation. ▪ •Many pollutant-degrading microorganisms are hidden in complex environmental microbial communities.•The culture-independent multi-omics approach provides information about the potential key microorganisms and functional genes in industrial saponification wastewater.•The multi-omics approach guides the design of a culture method to obtain the organohalide dehalogenation microorganism.