Molecular amplification and purification of the E. colt recC gene product

The level of recC gene expression has been analysed using Mud.(bla lac) fusions to the recC promoter. The constitutive level of expression is very low and remains so even under SOS inducing conditions. The recC gene product has been amplified by harnessing the gene to the phage lambda leftward promo...

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Published inNucleic acids research Vol. 12; no. 9; pp. 3807 - 3819
Main Authors Hickson, Ian D., Atkinson, Karen E., Hutton, Linda, Tomkinson, Alan E., Emmerson, Peter T.
Format Journal Article
LanguageEnglish
Published Oxford University Press 11.05.1984
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Summary:The level of recC gene expression has been analysed using Mud.(bla lac) fusions to the recC promoter. The constitutive level of expression is very low and remains so even under SOS inducing conditions. The recC gene product has been amplified by harnessing the gene to the phage lambda leftward promoter in a plasmid. From cells harbouring this plasmid, RecC protein, which represented approximately 6% of the total cellular protein, was purified to eleotrophoretic homogeneity.
Bibliography:ArticleID:12.9.3807
1Present address: Department of Clinical Oncology, University of Newcastle upon Tyne, Royal Victoria Infirmary, Newcastle upon Tyne NE1 4LP, UK
istex:AC6D077B370486FD7497B937ED105356DD6CB61A
ark:/67375/HXZ-BR5GC2FM-F
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/12.9.3807