Proteomics for Apoptosis

• Published in: SEIBUTSU BUTSURI KAGAKU Vol. 47; no. 4; pp. 137 - 141
• Main Authors: Kuramitsu, Yasuhiro, Fujimoto, Masanori, Nakamura, Kazuyuki
• Format: Journal Article
• Language: English
• Published: Japanese Electrophoresis Society 2003
• Subjects: 2DE; apoptosis; PEBP; proteome; proteomics
• ISSN: 0031-9082; 1349-9785
• DOI: 10.2198/sbk.47.137

In these days proteome analyses are performed in the field of apoptosis. There are two methods to identify the proteins related to apoptosis. The first is to identify the proteins whose expression change after adding the stimuli which induce apoptosis in cells. The second is to compare 2DE pattern of intracellular proteins from two cell lines, one is sensitive against the inducer to apoptosis, and the other is resistant against this. We used these two methods and identified some proteins which may be related to apoptosis. For the first, heat shock (45°C, 30min) induced the over expression of stathmin and thymosin β-4 in human T cell line Jurkat, and decrease of eukaryotic initiation factor 5A in human pancreatic cancer cell line MIA PaCa-2. For the second, we established TNF-α-resistant cell line cKDH-8/11 and sensitive cell line KDH-8/YK from rat hepatoma cell line KDH-8. From differential display using 2-DE, we detected spot 1 which expressed in only KDH-8/YK, and spot 2 whose expression is increased in cKDH-8/11. The spot 2 was identified as phosphatidylethanolamine-binding protein (PEBP).