High Throughput Primer Walking of cDNA Clones

Primer walking of cloned DNA is a standard research tool. It has been used in the past to determine the sequence of individual clones of interest. With the expansion of DNA sequencing capacity the need to be able to walk larger numbers of clones has become necessary. Our laboratory is a mid-sized ge...

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Bibliographic Details
Published inJournal of Laboratory Automation Vol. 8; no. 2; pp. 64 - 65
Main Authors Stewart, Claudia, Lum, Nicole, Hill, Robert, Stephens, Robert, Rudnick, Paul, Rassmussen, Lynn, Munroe, David
Format Journal Article
LanguageEnglish
Published Los Angeles, CA SAGE Publications 01.04.2003
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Summary:Primer walking of cloned DNA is a standard research tool. It has been used in the past to determine the sequence of individual clones of interest. With the expansion of DNA sequencing capacity the need to be able to walk larger numbers of clones has become necessary. Our laboratory is a mid-sized genomics facility. In conjunction with the Advanced Biomedical Computing Center (ABCC) we have developed methods for automating the primer selection, DNA sequencing, contig assembly and sequence analysis for clones arrayed in microtiter format. This approach has allowed us to walk 475 clones (five microtiter plates) selected from a cDNA library.
ISSN:2211-0682
1535-5535
1540-2452
DOI:10.1016/S1535-5535-04-00256-4