Proteomic analysis of Morus leaf epidermis indicates the roles of photosystems and ROS in UV-B response

The epidermis is the first layer of cells on leaves that receive UV-B radiation and the place where guard cells distribute. Their stress response mechanism was different from the mesophyll. However, there has been little research on leaf epidermis. To assess the response mechanism of epidermis to UV...

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Bibliographic Details
Published inIndustrial crops and products Vol. 188; p. 115683
Main Authors Li, Yaohan, Xia, Haodong, Shawky, Eman, Liu, Shengzhi, Tao, Minglei, Liu, Amin, Sulaiman, Kaisa, Tian, Jingkui, Zhu, Wei
Format Journal Article
LanguageEnglish
Published Elsevier B.V 15.11.2022
Subjects
Calcium ion signaling
Epidermal
Light-reaction
Morus
UV-B radiation
NADPH
UVB
UVD
PS
PGAK
ANT
APX
H2DCFDA
PRM
HS
UV-B radiation
RuBisCo
TPI
CaM
SNARE
NDPK
Prx
FRAP
NADK
UV
CML
POD
MS
Epidermal
GO
SOD
DHAR
SYP
MAPK
ER
DEP
GAPD
Light-reaction
TEAC
NOX
CAT
ROS
PLCD
VDAC
Calcium ion signaling
Morus
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Summary:The epidermis is the first layer of cells on leaves that receive UV-B radiation and the place where guard cells distribute. Their stress response mechanism was different from the mesophyll. However, there has been little research on leaf epidermis. To assess the response mechanism of epidermis to UV-B radiation, tissue-specific proteomics were performed on the epidermis of Morus leaf under UV-B radiation and UV-B radiation following dark incubation. A total of 935 proteins were identified and quantified. Changes of proteins involved in light reaction and Calvin cycle were not simultaneous. Antioxidant enzymes showed different patterns. And the contents of H2O2 and Ca2+ were increased after treatments. A UV-B radiation response mechanism of epidermis was proposed based on these results, in which photosystems perceives the stimulus of UV-B radiation to produce reactive oxygen species, and then the antioxidant system and protein homeostasis system were induced through the Ca2+ crosstalk with reactive oxygen species signaling pathway. [Display omitted] •Tissue-specific proteomic analysis was performed on epidermis of Morus leaf.•Photosystems of epidermis perceives stimulus of UV-B radiation.•Reactive oxygen species and Ca2+ were stimulated by UV-B radiation.
ISSN:0926-6690
1872-633X
DOI:10.1016/j.indcrop.2022.115683