A comparative anticancer analysis of Iron Oxide nanoparticles of Hippophae rhamnoides and Cichorium intybus found in the Karakoram Range of Gilgit Baltistan against liver cancer targeting the RhoA gene

• Published in: Drug development and industrial pharmacy p. 1
• Main Authors: Tabassum, Rukhsana, Dilshad, Erum
• Format: Journal Article
• Language: English
• Published: England 10.09.2024
• Subjects: Rho A gene; liver cancer; Hippophae rhamnoides; Cichorium intybus; medicinal plants
• ISSN: 1520-5762
• DOI: 10.1080/03639045.2024.2400209

ObjectiveThe current research work focused on the evaluation of of and Fe O NPs against liver cancer cell line (HepG2) by performing antiproliferative assay targeting the gene and apoptotic pathway genes and proteins.MethodsFe O NPs were synthesized using extracts of and and characterized by UV-Vis spectroscopy, FTIR, SEM/EDS and XRD. MTT assay was used to study cytotoxicity against the HepG2 cells. Real-time qPCR and ELISA were used for the gene and protein analysis.ResultsAn absorbance peak at 300 nm for and 289 nm for nanoparticles were observed by UV-Vis analysis. The FTIR bands of Fe O NPs suggested the presence of aldehydes, alcohols and polyols whereas bands of Fe O NPs suggested the presence of carboxyl groups, hydroxyl groups, alkynes and amines. The size of Fe O NPs was found to be 27 ± 5nm for and 84 ± 4nm for The IC value of 41.69 µM for and 71.04 µM for Fe O NPs compared to plant extract (78.10 µM and 96.03 µM for and respectively were found against HepG2 cells. The gene expression and protein levels of were decreased whereas those of and were found increased.ConclusionNanoparticles and extract of were found more effective as compared to which was evident by the results of cytotoxicity and analysis of studied genes and proteins.