Exosomes isolated from citrus lemon: a promising candidate for the treatment of Alzheimer's disease

• Published in: Therapeutic delivery Vol. 15; no. 7; p. 507
• Main Authors: Dolma, Lobzang, Damodaran, Aswin, Panonnummal, Rajitha, Nair, Sreeja C
• Format: Journal Article
• Language: English
• Published: England 02.07.2024
• Subjects: Alzheimer Disease - drug therapy; Alzheimer Disease - metabolism; Antioxidants - pharmacology; Blood-Brain Barrier - metabolism; Cell Line, Tumor; Cell Survival - drug effects; Citrus - chemistry; Exosomes - metabolism; Humans; Oxidative Stress - drug effects; Particle Size; Permeability; beta-amyloid; SHSY-5Y cells; antioxidant; Alzheimer's disease; citrus lemon; exosomes like nanovesicles
• ISSN: 2041-6008
• DOI: 10.1080/20415990.2024.2354119

To investigate the efficacy of exosome-like nanovesicles from citrus lemon (EXO-CLs) in combating oxidative stress associated with Alzheimer's disease. EXO-CLs were isolated through differential ultracentrifugation, characterized for particle size and evaluated for antioxidant activity. EXO-CLs exhibited a mean size of 93.77 ± 12.31 nm, demonstrated permeability across the blood-brain barrier (BBB) and displayed antioxidant activity comparable to ascorbic acid. Additionally, they were found to be non-toxic, with over 80% cell viability observed in SH-SY5Y cells. The study proposes that EXO-CLs could serve as an effective treatment for neurodegenerative diseases. This suggests a promising approach for targeted interventions in brain-related disorders, owing to the antioxidant properties and BBB permeability exhibited by EXO-CLs.