The development of a new adjuvant lipid-saponin complex and its use at experimental immunization by bacterial antigen

• Published in: Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry Vol. 1; no. 4; pp. 353 - 358
• Main Authors: Tsybulsky, A. V., Sanina, N. M., Lee, I. A., Popov, A. M., Kostetsky, E. Ya, Portnyagina, O. Yu, Shnyrov, V. L.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01.12.2007
• Subjects: Bacteria; Freund's adjuvant; Glycolipids; glycosides; Immune response (humoral); ISCOMS; Lecithin; Liposomes; Macrophytes; Membrane proteins; Pores; Porins; Pseudotuberculosis; Saponins; Toxicity; triterpenes; Vaccination
• ISSN: 1990-7508; 1990-7516
• DOI: 10.1134/S1990750807040117

Immunostimulating complexes (ISCOM) have been modified by replacement of structural components of ISCOM; phosphatidylcholine has been replaced, by glycolipid of monogalactosyldiacylglycerol from sea macrophytes, saponin QuilA has been replaced by triterpene glycoside, Cucumarioside A^sub 2^-2. The resultant complex includes morphological structures of two types: the ISCOM-like structures with the characteristic morphology and sizes and also the tubular structures with diameter of about 40 nm and length of 150-400 nm. We have defined these structures as TI-complexes. These TI-complexes exhibit considerably lower toxicity than ISCOM. They may include an amphiphilic protein antigen and provide immunoadjuvant effect during experimental vaccination. Under conditions of the experimental immunization of mice with a weak immunogen (subunit membrane pore protein from Y. pseudotuberculosis), TI-complexes with antigen provided stronger humoral immune response to antigen than the complexes of porin with classical ISCOM, liposomes and Freund's adjuvant. Thus, the TI-complexes represent a new type of adjuvant carriers for antigens.[PUBLICATION ABSTRACT]