Capillary electrophoretic and liquid chromatographic determination of synthetic dyes in non-alcoholic drinks and wine samples

Synthetic dyes in wine, juices, lemonades, instant and non-alcoholic drinks were determined by capillary electrophoresis (CE) and liquid chromatography (HPLC) with UV-VIS diode-array detection (DAD). The results were in the very good agreement (the relative deviations delta16 %, delta14 % and delta1...

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Bibliographic Details
Published inCzech Journal of Food Sciences Vol. 18; no. 2; pp. 41 - 48
Main Authors Jancarova, I, Vondrackova, A, Slampova, A, Kuban, V. (Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic)
Format Journal Article
LanguageEnglish
Published Prague Czech Academy of Agricultural Sciences (CAAS) 01.01.2000
Czech Academy of Agricultural Sciences
Subjects
Acetonitrile
Alcoholic beverages
BOISSON NON ALCOOLISEE
Buffers
Capillary electrophoresis
COLORANT ALIMENTAIRE
COLORANTES ALIMENTARIOS
Dyes
ELECTROFORESIS
ELECTROPHORESE
ELECTROPHORESIS
ESPECTROMETRIA
FOOD COLOURANTS
FRUIT JUICES
GASEOSAS
High performance liquid chromatography
HPLC
JUGO DE FRUTAS
JUS DE FRUITS
Liquid chromatography
non-alcoholic drinks
pH effects
Separation
SOFT DRINKS
SPECTROMETRIE
SPECTROMETRY
Spectrophotometry
synthetic dyes
VIN
VINOS
Wine
WINES
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Summary:Synthetic dyes in wine, juices, lemonades, instant and non-alcoholic drinks were determined by capillary electrophoresis (CE) and liquid chromatography (HPLC) with UV-VIS diode-array detection (DAD). The results were in the very good agreement (the relative deviations delta16 %, delta14 % and delta15 % between CE/UV-VIS, CE/HPLC and HPLC/UV-VIS, respectively, D 6 % in majority of samples) with those obtained by UV-VIS spectrophotometry. A borate/phosphate buffer of pH 9.0 (12.5mM borate and 12.5mM phosphate) containing 40mM sodium dodecylsulfate (SDS) was selected as an electrolyte for CE. A 50mM phosphate buffer and 5mM tetrabutylammonium hydroxide (TBAOH) water solution of pH 4.2 in 38 %, (v/v) acetonitrile were used as a mobile phase for isocratic HPLC separation on Sepharon SGX C18 (3x150 mm, 5 microm) column. RSDs for individual colorants were 0.5 % for migration times, 3 %, for electrophoretic peak areas, 0.9 % for retention times and 2.4 %, for HPLC peak areas. Limits of quantitations (LOQs for 10 S/N) were 1.5-3.3 mg/l for CE when using 50 microm I.D. separation capillary or 0.2 to 0.4 mg/l for HPLC.
Bibliography:Q04
2000000885
Q05
ISSN:1212-1800
1805-9317
DOI:10.17221/8307-CJFS