A comparative analysis of stomata and leaf trichome characteristics in Quercus robur L. genotypes

• Published in: Zbornik Matice Srpske za Prirodne Nauke Vol. 2003; no. 105; pp. 51 - 59
• Main Authors: Nikolic, Natasa, Merkulov, Ljiljana, Krstic, Borivoj, Orlovic, Sasa
• Format: Journal Article
• Language: English
• Published: Matica srpska 2003
• Subjects: genotype variability; leaf surface; Quercus robur; stomata; trichomes
• ISSN: 0352-4906; 2406-0828
• DOI: 10.2298/ZMSPN0305051N

The objective of this study was to determine genotype variability of leaf trichome and stoma characteristics. Leaves were sampled from seventeen pedunculate oak (Quercus robur L) genotypes originating from clonal seed orchard Banov Brod (Srem, the Vojvodina Province). The pedunculate oak has hypostomatal leaves. Statistically significant differences were found for the dimensions and density of stomata. Genotype variability of stomatal dimensions was less pronounced in comparison with their density (CV = 8.88%). Stomata number ranged from 530 to 791 per mm2 of leaf area; genotypes 18 and 25 could be distinguished from the others for the highest stomata number per leaf unit area, genotype 35 for the lowest number. In all genotypes, only solitary eglandular trichomes were observed on the adaxial leaf surface while both solitary eglandular and uniseriate glandular hairs were present on the abaxial surface. Single glandular trichomes were observed in all genotypes, while some of them were characterized by the presence of two (genotypes 4, 5, 6, 16, 22, 25, 28, 29, 30, 35, 38, 40, and 85) or three (genotypes 16, 25, 35) hairs joined by their basal cells. U radu su poucavane karakteristike stoma i lisnih trihoma u cilju utvrdjivanja genotipske varijabilnosti. Uzorci listova su uzeti sa sedamnaest genotipova hrasta luznjaka (Quercus robur L), koji poticu iz vegetativne semenske plantaze Banov Brod (Srem, Vojvodina). Hrast luznjak ima hipostomatalne listove. Utvrdjene su statisticki znacajne razlike za dimenzije i broj stoma. Genotipska varijabilnost duzine i sirine stoma bila je manje porazena u poredjenju sa njihovim brojem (CV = 8.88%). Broj stoma po jedinici povrsine lista se kretao od 530 do 791. Genotipovi 18 i 25 su se izdvajili po najvecem, a genotip 35 po najmanjem broju stoma. Kod svih genotipova, na adaksijalnoj povrsini lista utvrdjeno je prisustvo nezlezdanih trihoma solitarnog tipa, dok su na abaksijalnoj, pored nezlezdanih, prisutne i proste-jednonizne zlezdane dlake. Pojedinacne zlezdane dlake su najmnogobrojnije i prisutne su kod svih genotipova. Neki od njih se karakterisu prisustvom dve (genotipovi 4, 5, 6, 16, 22, 25, 28 29, 30, 35, 38, 40, 85) ili tri (genotipovi 16, 25, 35) zlezdane dlake, koje su spojene svojim bazalnim delom.