Antifungal activity of essential oil Hyssopus officinalis L. against micopathogen Mycogone perniciosa (Mang)

• Published in: Zbornik Matice Srpske za Prirodne Nauke Vol. 2005; no. 109; pp. 123 - 128
• Main Authors: Glamoclija, Jasmina, Sokovic, Marina, Vukojevic, Jelena, Milenkovic, Ivanka, Brkic, Dejan, Van, Griensven
• Format: Journal Article
• Language: English
• Published: Matica srpska 2005
• Subjects: Agaricus bisporus; antifungal activity; essential oil composition; Hyssopus officinalis; Mycogone perniciosa
• ISSN: 0352-4906; 2406-0828
• DOI: 10.2298/ZMSPN0519123G

The most commonly cultivated mushroom species is the Agaricus bisporus Lange (Imb). One of the major pathogenic diseases of the cultivated mushroom in Serbia is Mycogone perniciosa (Mang). Biological control systems are not much used in mushroom cultivation. Medical and aromatic plants have been placed in the focus of intense studies. Pure culture of the M. perniciosa was isolated from infected A. bisporus. The essential oil of Hyssopus officinalis L. is used as a potential antifungal agent. The most abundant components in oil are isopinocamphone (43.29%), pinocamphone (16.79%) and b-pinene (16.31%). Antifungal activity of Hyssop was investigated by the modified microatmosphere method. The minimal inhibitory quantity was 5 ?L/mL and a minimal fungicidal quantity was 15-20 ?L/mL. There is no report on the use of Hyssop essential oil in mushroom disease. Agaricus bisporus Lange (Imb) je najcesce komercijalno gajena jestiva gljiva Razliciti mikroorganizmi gljive, bakterije i virusi su izazivaci bolesti u gajilistima sampinjona. Mycogone perniciosa (Mang) je izazivac bolesti poznate pod nazivom vlazni mehur i najcesci uzrocnik gubitaka u gajilistima u Srbiji. Bioloska kontrola, koja je uspesno primenjivana na nekim poljoprivrednim kulturama, nije koriscena prilikom uzgoja gljiva. Jedna od mogucnosti je primena biljnih sprejova. Lekovite i aromaticne vrste biljaka se intenzivno istrazuju kao moguci antifungalni agensi. Uzorci obolelih sampinjona su sakupljani u gajilistima u Srbiji. Kulture M. perniciosa su izolovane sa obolelih plodonosnih tela A. bisporus. Korisceno je etarsko ulje Hyssopus officinalis. Najzastupljenije komponente ulja su izopinokamfon (43.29%) trans-pinokamfon (16.79%) i b-pinen (16.31%). Antifungalna aktivnost etarskog ulja izopa ispitivana je modifikovanom "mikroatmosfera"- metodom. Minimalna inhibitorna kolicina je bila 5 ?L/mL, a minimalna fungicidna kolicina 15-20 ?L/mL. Velik broj preparata je napravljen i primenjen za kontrolisanje oboljenja pecuraka: fungicidi, primena mikrotalasa ili dejstvo nekih antagonistickih bakterija. Dosad nije bilo saopstenja o primeni etarskog ulja izopa protiv izazivaca bolesti gajenih gljiva.