Downregulation of miR‐1270 mediates endothelial progenitor cell function in preeclampsia: Role for ATM in the Src/VE‐cadherin axis

• Published in: The FASEB journal Vol. 36; no. 7; pp. e22379 - n/a
• Main Authors: Schröder‐Heurich, Bianca, Büder, Tim, Meyer, Nadia, Vu, Thu Huong, Richter, Katja, Ramachandran, Dhanya, Brodowski, Lars, Kaisenberg, Constantin S., Versen‐Höynck, Frauke
• Format: Journal Article
• Language: English
• Published: United States 01.07.2022
• Subjects: ATM; cardiovascular risk; ECFC; endothelial progenitor cells; miR‐1270; preeclampsia; VE‐cadherin; endothelial progenitor cells; VE-cadherin; ATM; ECFC; cardiovascular risk; preeclampsia; miR-1270
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fj.202200040RR

Preeclampsia, a pregnancy‐related hypertensive disorder, is associated with endothelial dysfunction and increased cardiovascular risk of the offspring in adulthood. In preeclampsia, endothelial colony‐forming cells (ECFC) are reduced in number and function. Recently, we have shown that miR‐1270, which is involved in cancer in vitro proliferation, migration, and tumor progression, is downregulated in fetal ECFC from preeclamptic pregnancies. We now hypothesize that miR‐1270 dysregulation contributes to vascular endothelial dysfunction occurring after preeclampsia via ATM (ataxia telangiectasia mutated) overexpression, the key kinase of DNA damage repair. Here, we show that miR‐1270 silencing in normal ECFC and downregulation in preeclamptic ECFC are accompanied by an increase in the expression levels of ATM. Furthermore, ATM activation correlates with upregulated tyrosine kinase Src leading to phosphorylation and internalization of VE‐cadherin (vascular endothelial‐cadherin) which subsequently compromises endothelial barrier permeability and morphodynamic cell parameters. Treatment with specific ATM inhibitors reveals a novel role of ATM upstream of tyrosine kinase Src activation. Subsequently, Src phosphorylation and internalization of VE‐cadherin compromise endothelial barrier permeability. Our findings suggest that downregulation of miR‐1270 contributes to impaired ECFC function via the associated ATM overexpression, which further identifies ATM as a novel and critical factor for ECFC defects in preeclampsia. Our study provides new insights into the understanding of ECFC impairment associated with cardiovascular risk in preeclamptic offspring and identifies potential novel therapeutic targets.