Nuclear transfer of goat somatic cells transgenic for human lactoferrin gene

Transgenic animal mammary gland bioreactors are used to produce recombinant proteins with appropriate post-translational modifications. The nuclear transfer of transgenic somatic cells is a powerful method to produce mammary gland bioreactors. We established an efficient gene transfer and nuclear tr...

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Bibliographic Details
Published inFrontiers of biology in China Vol. 3; no. 3; pp. 269 - 274
Main Authors Li, Lan, Shen, Wei, Min, Lingjiang, Pan, Qingyu, Sun, Yujiang, Deng, Jixian, Pan, Qingjie
Format Journal Article
LanguageEnglish
Published Heidelberg SP Higher Education Press 01.09.2008
Subjects
Biochemistry
Biomedical and Life Sciences
Cell Biology
Developmental Biology
Genetics and Population Dynamics
Life Sciences
Neurobiology
Proteomics
Research Article
human lactoferrin
goat
gene transfer
mammary gland bioreactor
nuclear transfer
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Summary:Transgenic animal mammary gland bioreactors are used to produce recombinant proteins with appropriate post-translational modifications. The nuclear transfer of transgenic somatic cells is a powerful method to produce mammary gland bioreactors. We established an efficient gene transfer and nuclear transfer approach in goat somatic cells. Gene targeting vector pGBC2LF was constructed by cloning human lactoferrin (LF) gene cDNA into exon 2 of the milk goat beta-casein gene and the endogenous start codon was replaced by that of human LF gene. Goat fetal fibroblasts were transfected with linearized pGBC2LF and 14 cell lines were positive according to PCR and Southern blot. The transgenic cells were used as donor cells of nuclear transfer and some of reconstructed embryos could develop into blastocyst in vitro.
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ISSN:1673-3509
1673-3622
DOI:10.1007/s11515-008-0052-8