Suitable unialgal strains of Gracilariopsis chorda and Gracilaria vermiculophylla for hemagglutinin production
The selection of unialgal culture strains suitable as sources of algal hemagglutinins is one of the important factors for success in algal component production. To obtain suitable unialgal culture strains, several Gracilariopsis chorda and Gracilaria vermiculophylla strains were surveyed for hemaggl...
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Published in | Journal of applied phycology Vol. 32; no. 4; pp. 2397 - 2406 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.08.2020
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | The selection of unialgal culture strains suitable as sources of algal hemagglutinins is one of the important factors for success in algal component production. To obtain suitable unialgal culture strains, several
Gracilariopsis chorda
and
Gracilaria vermiculophylla
strains were surveyed for hemagglutinating activity, relative growth rates, and fertility difficulty. Fertile tetrasporophyte samples of naturally occurring
G. chorda
and
G. vermiculophylla
were collected at each of three different collection sites around Shikoku Island in southwest Japan. Unialgal culture strains were started from isolated tetraspores obtained from each naturally occurring plant. The hemagglutinating activities in algal extracts of the three
G. chorda
strains (3100–7000 units mg
−1
) were higher than those of the three
G. vermiculophylla
strains (920–980 units mg
−1
). The daily growth rates of the
G. chorda
strains (8.0–13.8% day
−1
at 22 °C) were higher than those of the
G. vermiculophylla
strains (4.1–4.4% day
−1
at 22 °C). Among the unialgal culture strains tested, the one started from isolated tetraspores from
G. chorda
growing in the Katsuura River had the highest hemagglutinating activity and relative growth rate. This strain also did not become fertile even after a period of 3 years of culture with aeration at 22 °C, in a 14-h light–10-h dark cycle at 60 μmol photons m
−2
s
−1
. Thus, the unialgal culture strain started from isolated tetraspores from
G. chorda
growing in the Katsuura River seems to be a useful source for hemagglutinin production. |
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ISSN: | 0921-8971 1573-5176 |
DOI: | 10.1007/s10811-019-02024-z |