A dual-channel fusion sensing platform of multicolor polyaniline visual and self-powered photoelectrochemical detection of mcirocystin-RR

• Published in: Microchemical journal Vol. 204; p. 111097
• Main Authors: Du, Xiaojiao, Zhu, Rongquan, Zhang, Bing, Ji, Xingyu, Jiang, Xiaoyan, Lian, Yuebin, Sun, Jun
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2024
• Subjects: Dual-channel; MC-RR; Self-powered aptasensor; Visualization; Visualization; Dual-channel; Self-powered aptasensor; MC-RR
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2024.111097

This work was dual-channel MC-RR sensor involved and allowed for not only quantitative detection using electrical signals, but also sensitive visual analysis via multicolor changes. [Display omitted] •A multicolor visualized self-powered photoelectrochemical sensing platform was proposed.•The proposed dual-channel strategy provided the diversity of signal readouts.•The platform exhibited the excellent detection accuracy, selectivity, and stability.•This sensor was applied for detection of microcystin-RR in lake and serum samples. The focus of this work was to develop a novel dual-channel platform based on the multi-color electrochromic visualization and self-powered photoelectrochemical sensors for detection of microcystin-RR (MC-RR). On the one hand, utilizing the inhibited output performance of the sensing platform by MC-RR which was captured by the aptamer, the MC-RR concentration was quantified via the short-circuit current (Isc) value of the proposed sensing platform. On the other hand, the transfer of photoelectrons produced by the g-C3N4/ZnIn2S4 photoanode to the modified polyaniline (PANI) photocathode produced a multi-color change from bluish violet to yellow-green, which was used to achieve visual signal readouts. Under optimal conditions, the Isc and the average color channel values showed a linear relationship with the logarithm of MC-RR concentration in the range of 10-4 ∼ 102 nM and 5 × 10-4 ∼ 102 nM, with the detection limits of ∼ 3.3 × 10-5 nM and ∼ 1.7 × 10-4 nM (S/N = 3), respectively. Meanwhile, the proposed dual-channel visualized self-powered platform had excellent reproducibility and stability, providing a prototype for the detection of MC-RR in real samples. The proposed sensing platform not only provided the diversity of signal readouts, but also enhanced the reliability and accuracy of the detection.