Catalytic Properties of a Nanozyme Based on Silver Nanoparticles Immobilized into a Polymethacrylate Matrix

• Published in: Kinetics and catalysis Vol. 66; no. 2; pp. 232 - 239
• Main Authors: Bragina, S. K., Gavrilenko, N. A., Saranchina, N. V., Gavrilenko, M. A.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.04.2025; Springer Nature B.V
• Subjects: Catalysis; Chemistry; Chemistry and Materials Science; Hydrogen peroxide; Indigo; Nanocomposites; Nanoparticles; Oxidation; Peroxidase; Physical Chemistry; Polymethacrylates; Silver; Thermal reduction; polymethacrylate; nanozyme; silver nanoparticles; peroxidase-like activity; glucose detection
• ISSN: 0023-1584; 1608-3210
• DOI: 10.1134/S002315842560035X

The catalytic peroxidase-like properties of silver nanoparticles (Ag NPs) immobilized into a polymethacrylate matrix (PMM) were studied. The Ag NPs were prepared by thermal reduction of silver cations preimmobilized in a PMM. The morphology of the nanocomposite was studied using scanning electron microscopy (SEM), and the average size of the synthesized individual spherical nanoparticles was 18 ± 5 nm. It was demonstrated that silver nanoparticles immobilized in a polymethacrylate matrix (PMM–Ag 0 ) exhibited pronounced peroxidase-like activity in the oxidation reaction of a chromogenic substrate, indigo carmine, under the action of H 2 O 2 . The Michaelis–Menten model was used to estimate the kinetic parameters of the reaction. The Michaelis constants ( K m ) of 0.1 and 1.0 mM for indigo carmine and H 2 O 2 , respectively, indicated a strong affinity of the substrates to silver nanoparticles in PMM.