Identification of Candida haemulonii Complex Species: Use of ClinProToolsTM to Overcome Limitations of the Bruker BiotyperTM, VITEK MSTM IVD, and VITEK MSTM RUO Databases
Candida haemulonii is now considered a complex of two species and one variety: C. haemulonii sensu stricto, Candida duobushaemulonii and the variety C. haemulonii var. vulnera . Identification (ID) of these species is relevant for epidemiological purposes and for therapeutic management, but the diff...
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Published in | Frontiers in microbiology Vol. 7 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
16.06.2016
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Subjects | |
Online Access | Get full text |
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Summary: | Candida haemulonii
is now considered a complex of two species and one variety:
C. haemulonii
sensu stricto,
Candida duobushaemulonii
and the variety
C. haemulonii
var.
vulnera
. Identification (ID) of these species is relevant for epidemiological purposes and for therapeutic management, but the different phenotypic commercial systems are unable to provide correct species ID for these emergent pathogens. Hence, we evaluated the MALDI-TOF MS performance for the ID of
C. haemulonii
species, analyzing isolates/strains of
C. haemulonii
complex species,
Candida pseudohaemulonii
and
Candida auris
by two commercial platforms, their databases and softwares. To differentiate
C. haemulonii
sensu sctricto from the variety
vulnera
, we used the ClinProTools
TM
models and a single-peak analysis with the software FlexAnalysis
TM
. The Biotyper
TM
database gave 100% correct species ID for
C. haemulonii
sensu stricto,
C. pseudohaemulonii
and
C. auris
, with 69% of correct species ID for
C. duobushaemulonii
. Vitek MS
TM
IVD database gave 100% correct species ID for
C. haemulonii
sensu stricto, misidentifying all
C. duobushaemulonii
and
C. pseudohaemulonii
as
C. haemulonii
, being unable to identify
C. auris
. The Vitek MS
TM
RUO database needed to be upgraded with in-house SuperSpectra to discriminate
C. haemulonii
sensu stricto, C.
duobushaemulonii
,
C. pseudohaemulonii
, and
C. auris
strains/isolates. The generic algorithm model from ClinProTools
TM
software showed recognition capability of 100% and cross validation of 98.02% for the discrimination of
C. haemulonii
sensu stricto from the variety
vulnera.
Single-peak analysis showed that the peaks 5670, 6878, or 13750 m/z can distinguish
C. haemulonii
sensu stricto from the variety
vulnera. |
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Bibliography: | Reviewed by: Lucilla Iacumin, University of Udine, Italy; Masoomeh Shams-Ghahfarokhi, Tarbiat Modares University, Iran; Yunlong Li, Wadsworth Center, USA Edited by: Fabrice Merien, Auckland University of Technology, New Zealand This article was submitted to Infectious Diseases, a section of the journal Frontiers in Microbiology |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2016.00940 |