Natural Infection of Murraya paniculata and Murraya sumatrana with ' Candidatus Liberibacter asiaticus' in Java

The phloem-limited bacterium ' Liberibacter asiaticus' ( Las) is the putative causal pathogen of the severe Asiatic form of huanglongbing (citrus greening) and is most commonly transmitted by the Asiatic citrus psyllid . Las severely affects many species and hybrids and has been recorded i...

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Published inPlant disease Vol. 108; no. 9; p. 2760
Main Authors Lestiyani, Ayu, Joko, Tri, Holford, Paul, Charles Beattie, George Andrew, Donovan, Nerida, Mo, Jianhua, Subandiyah, Siti, Iwanami, Toru
Format Journal Article
LanguageEnglish
Published United States 01.09.2024
Subjects
Murraya lucida
Murraya paniculata
Candidatus Liberibacter asiaticus
host plant
Murraya omphalocarpa
phylogeny
Murraya sumatrana
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Summary:The phloem-limited bacterium ' Liberibacter asiaticus' ( Las) is the putative causal pathogen of the severe Asiatic form of huanglongbing (citrus greening) and is most commonly transmitted by the Asiatic citrus psyllid . Las severely affects many species and hybrids and has been recorded in the relative, orange jasmine, (L.) Jack (syn. . L.). In this study, 13 accessions of three species ( , Roxb., and [G.Forst.] Mabb.) and the Papuan form of a putative hybrid ( Hayata) were identified morphologically and molecularly based on sequence identity of the region of the chloroplast genome, and infection on these plants under field conditions was determined by PCR and quantitative real-time PCR (qPCR) on two to four occasions over 14 months. Las was repeatedly detected in leaflet midribs by PCR and qPCR on four and three accessions of . and , respectively. It was not detected in leaflet midribs of single accessions of and . The species identification of the Las-positive accessions was further confirmed using all the molecular taxonomic markers consisting of the six fragments of the maternally inherited chloroplast genome and part of the nuclear-encoded internal transcribed spacer (ITS) region. The results indicated that natural infection of and with Las can occur in Java. To our knowledge, this is the first demonstration of the natural infection of with Las. Further studies are required to determine whether infections persist in the absence of .
ISSN:0191-2917
DOI:10.1094/PDIS-12-23-2593-RE