NANOSTRUCTURED FERRIC CITRATE EFFECT ON Chlorella vulgaris DEVELOPMENT

• Published in: Biotechnologia acta Vol. 11; no. 6; pp. 47 - 54
• Main Author: Golub, N. B.
• Format: Journal Article
• Language: English
• Published: Kyiv Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine 01.12.2018; National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
• Subjects: Algae; Biomass; Carotenoids; Cell culture; Chlorella; chlorella cells; Chlorella vulgaris; Chlorophyll; chlorophylls; Citric acid; Ferric citrate; Intracellular; intracellular iron; Iron; Nanostructure; nanostructured ferric citrate; Photosynthesis; Pigments; Sulfur
• ISSN: 2410-7751; 1995-5537; 2410-776X
• DOI: 10.15407/biotech11.06.047

The aim of the research was to study the development of Chlorella vulgaris at culturing on the modified Gromov 6 medium with high concentrations of nanostructured ferric citrate and also its effect on photosynthesis pigments accumulation. It was demonstrated that the highest intracellular iron content (15 mg/g of dry mass) in the culture cells was typical with nanostructured ferric citrate content of 30 mg/dm3 of culture medium, the highest content of chlorophyll a - 23 mg/g of dry algae mass, b - 7.5 mg/g of dry mass, and carotenoids - 9.2 mg/g of dry mass was observed at nanostructured ferric citrate content of 20 mg/dm3. The use of nanostructured ferric citrate leaded to an increase in the chlorella biomass yield by 3 times with compared to standard technology. Simultaneously, intracellular iron content in cells increased significantly with the use of nanostructured ferric citrate, which increases their value as a nutritional supplement. In order to increase the biomass yield and intracellular iron content in cells, application of nanostructured ferric citrate is recommended.