Dehydrins as stress proteins related to frost tolerance of barley

• Published in: Kvasný průmysl (On-line) Vol. 56; no. 2
• Main Authors: Holkova, L.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic), Mikulkova, P.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic), Hrstkova, P.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic), Prasil, I.T.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic), Bradacova, M.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic), Chloupek, O.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic)
• Format: Journal Article
• Language: English
• Published: 01.02.2010
• Subjects: BARLEY; CEBADA; COLD STRESS; COLD TOLERANCE; DEHYDRATION; DESHIDRATACION; DESHYDRATATION; DROUGHT STRESS; ESTRES DE FRIO; ESTRES DE SEQUIA; FROST RESISTANCE; GENE; GENES; GENETIC POLYMORPHISM; HORDEUM VULGARE; http://www.fao.org/aos/agrovoc#c_11025; http://www.fao.org/aos/agrovoc#c_16067; http://www.fao.org/aos/agrovoc#c_2162; http://www.fao.org/aos/agrovoc#c_24031; http://www.fao.org/aos/agrovoc#c_24993; http://www.fao.org/aos/agrovoc#c_3214; http://www.fao.org/aos/agrovoc#c_34079; http://www.fao.org/aos/agrovoc#c_3662; http://www.fao.org/aos/agrovoc#c_37531; http://www.fao.org/aos/agrovoc#c_6259; http://www.fao.org/aos/agrovoc#c_6520; http://www.fao.org/aos/agrovoc#c_8157; http://www.fao.org/aos/agrovoc#c_823; http://www.fao.org/aos/agrovoc#c_9994; ORGE; PCR; POLIMORFISMO GENETICO; POLYMORPHISME GENETIQUE; PROTEINAS; PROTEINE; PROTEINS; RESISTANCE A L'HIVER; RESISTANCE AUX FACTEURS NUISIBLES; RESISTANCE AUX GELEES; RESISTANCE TO INJURIOUS FACTORS; RESISTENCIA A AGENTES DANINOS; RESISTENCIA A LAS HELADAS; RESISTENCIA INVERNAL; STRESS DU A LA SECHERESSE; STRESS DU AU FROID; TOLERANCE AU FROID; TOLERANCIA AL FRIO; VARIEDADES; VARIETE; VARIETIES; WINTER HARDINESS
• ISSN: 0023-5830; 2570-8619
• DOI: 10.18832/kp2010010

Dehydrins are stress proteins related to frost and dehydration tolerance. The objective of this study was to determine the polymorphism of the dehydrin genes (Dhn) in relation to the frost tolerance in barley. Primers specific for Dhn4 and Dhn7 gene sequences were used for length polymorphism analysis in 30 varieties. PCR products showed two different matrices; the first (O type sequence) was specific for six-rowed winter and intermediate varieties characterised by high frost tolerance. It was typical of the Okal variety. The second type (A type sequence) was found only in two-rowed winter and spring varieties (e.g. Akcent) and was related to a lower level of frost tolerance. The length of the main polymorphic products corresponded to different alleles of the Dhn4 and Dhn7 genes. This difference in length was caused by the coincident presence of two specific mutations, i.e., an insertion of 6 bp in Dhn4 and a deletion of 30 bp in Dhn7 in O type barley varieties.