Antifungal activity of heat-treated wood extract against wood decay fungi

• Published in: International biodeterioration & biodegradation Vol. 193; p. 105843
• Main Authors: Bi, Zhenju, Gao, Xinqi, Zhang, Jing, Lei, Yafang, Yan, Li
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.08.2024
• Subjects: Antifungal activity; Cell membrane integrity; Enzymatic activity; Heat-treated wood extract; Wood decay resistance; Wood decay resistance; Antifungal activity; Heat-treated wood extract; Cell membrane integrity; Enzymatic activity
• ISSN: 0964-8305; 1879-0208
• DOI: 10.1016/j.ibiod.2024.105843

Using heat treatment for wood protection has been driven to maturity, but the role of heat-treated wood extracts in decay resistance has lacked attention. To assess the potential of heat-treated wood extract for wood preservation, the antifungal activity of the extract against wood decay fungi and the effects of the extract on fungal wood degrading enzyme activity and cell membrane integrity were tested. Small shavings generated during the processing of larch (Larix gmelinii (Rupr.) Kuzen) were heat-treated and extracted. The antifungal activity of extract against wood decay fungi and decay resistance of extract impregnated wood were tested to assess their potential. The effect of extracts on the enzyme activity of the white-rot fungus, Trametes versicolor (L.) Lloyd and the brown-rot fungus, Gloeophyllum trabeum (Pers.: Fr.) Murr. for wood degradation was assessed by detecting the activity of cellulose, hemicellulose, and ligninase of fungi incubated with extract. The effect of extracts on the integrity of cell membranes of fungi was assessed by staining with propidium iodide (PI) and the leakage detection of nucleic acid and protein in fungi after exposure to extract. The toxicity to freshwater luminescent bacteria (Vibrio qinghaiensis sp. -Q67) and mouse macrophages (RAW264.7) of the extract impregnated wood leachate was tested and compared with the leachate of the raw wood. The results denoted that the decay resistance of poplar (Populus tomentosa Carr.) wood could be improved by heat-treated larch wood extract, and the effect of the extract impregnated wood leachate on Q67 and RAW264.7 was the same as that of raw wood leachate. The extract inhibited ligninase activity (only for T. versicolor), cellulase activity, and respiratory metabolism of tested fungi, and impaired the membrane integrity. The study identified a potential wood preservative. [Display omitted] •Heat-treated wood extract improved the decay resistance of impregnated wood.•Extract disrupts cell membrane integrity of wood decay fungi.•Extract inhibits the activity of fungal wood degrading enzymes.•The use of heat-treated wood extract utilizes the wood processing by-products.