The Kinetics and Mechanism of Inhibition of Horseradish Peroxidase Activity by N-(Phosphonomethyl)glycine

The inhibition of horseradish peroxidase activity by N-(phosphonomethyl)glycine, the active ingredient of the Roundup herbicide (glyphosate), which is the most common herbicide in agricultural practice in the world, was investigated. It has been shown that N-(phosphonomethyl)glycine inhibited the ac...

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Bibliographic Details
Published inBiophysics (Oxford) Vol. 68; no. 3; pp. 347 - 355
Main Authors Avdeeva, L. V., Vakhterova, Ya. V., Saratovskikh, E. A.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.06.2023
Springer Nature B.V
Subjects
Biological and Medical Physics
Biophysics
Free radicals
Glycine
Glyphosate
Herbicides
Horseradish peroxidase
Molecular Biophysics
Physics
Physics and Astronomy
enzyme inhibition
Roundup (glyphosate)
horseradish peroxidase
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Summary:The inhibition of horseradish peroxidase activity by N-(phosphonomethyl)glycine, the active ingredient of the Roundup herbicide (glyphosate), which is the most common herbicide in agricultural practice in the world, was investigated. It has been shown that N-(phosphonomethyl)glycine inhibited the activity of peroxidase from horseradish roots by a competitive type, K i = 0.1004 mM. Michaelis constant was K m = 1.776 mM and the half-maximal inhibitory concentration was I 50 = 0.302 mM. The data we obtained indicated that N-(phosphonomethyl)glycine can block the work of the antioxidant systems and can cause the accumulation of free radicals in warm-blooded organisms, which is an addition to the available literature data on biological targets of Roundup.
ISSN:0006-3509
1555-6654
DOI:10.1134/S0006350923030028