On the Role of Ectonucleotidases in Synaptic Transmission. Thermolability of ATP Metabolism

ATP as an intermediate in the neuromuscular synapse can be released by quantum and non-quantum secretion from the motor neurons of the central and peripheral nervous system together with the main neurotransmitter, acetylcholine. Sequential hydrolytic dephosphorylation of ATP to adenosine in the syna...

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Bibliographic Details
Published inBiophysics (Oxford) Vol. 68; no. 4; pp. 576 - 580
Main Authors Khairullin, A. E., Efimova, D. V., Ivanova, D. V., Grishin, S. N., Ziganshin, A. U.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.08.2023
Springer Nature B.V
Subjects
Adenosine
Biological and Medical Physics
Biophysics
Biophysics of Complex Systems
Dephosphorylation
Metabolism
Metabolites
Motor neurons
Nervous system
Neuromodulation
Neuromuscular junctions
Physics
Physics and Astronomy
Skeletal muscle
Synaptic cleft
Synaptic transmission
synaptic signaling
neuromuscular synapse
ectonucleotidase
ATP metabolism
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Summary:ATP as an intermediate in the neuromuscular synapse can be released by quantum and non-quantum secretion from the motor neurons of the central and peripheral nervous system together with the main neurotransmitter, acetylcholine. Sequential hydrolytic dephosphorylation of ATP to adenosine in the synaptic cleft is a cascade of reactions catalyzed by ectonucleotidases. The study of the contribution of these enzymes to a complex signaling cascade is of great interest, since it is known about their dual function, limiting the action of ATP as a neurotransmitter and participation in the formation of other neuromodulators (ADP, AMP and adenosine); in addition, it is known that ATP and its metabolites can cause completely different physiological effects. The activity of ectonucleatidases in various types of skeletal muscles was determined in this study; it was shown that it varied within 5–7 IU. Lowering the temperature to 22°C led to a multiple decrease in the enzymatic metabolism of ATP in skeletal muscle tissues. It was assumed that the enzymatic limitation of ATP and the production of its metabolites ensures the stabilization of effective cholinergic neuromuscular transmission in normal and pathological conditions.
ISSN:0006-3509
1555-6654
DOI:10.1134/S0006350923040097