Molecular diagnosis of newcastle disease Iraqi virulent strain virus HN gene by specific primers design

• Published in: Kufa Journal For Veterinary Medical Sciences Vol. 5; no. 2; pp. 196 - 203
• Main Authors: al-Shammari, Ahmad Majid Hamzah, Hasan, Murtada Abd al-Mahdi Muhammad, al-Nasrawi, Huda Abd al-Hadi Ali
• Format: Journal Article
• Language: English
• Published: Najaf, Iraq University of Kufa, Faculty of Veterinary Medicine 31.12.2014; Faculty of Veterinary Medicine, University of Kufa
• Subjects: Chicken egg embryos; electrophoresis; NDV; Newcastle disease; Newcastle disease virus; Poultry; RT-PCR; specific primers; Virus diseases; الأمراض الفيروسية; الدواجن
• ISSN: 2077-9798; 2959-8478
• DOI: 10.36326/kjvs/2014/v5i24188

Newcastle disease is considered the most contagious poultry disease and may cause severe economic loss in the poultry industry, The first report in which NDV was consider as zoonotic disease and a human pathogen that was published by Burnet, in 1943. The aims of this study was to detect HN gene of two NDV Iraqi isolates (Najaf APMV1/ Chicken/ Iraq-najaf/ ICCMGR/2012and Baghdad ICCMGR) by designing specific priemrs for the Iraqi isolates. DNV isolates propagated in embryonated chicken eggs and showed to kill embryos in different times intervals ( more than 40 hours and less than 72 hours) with marked sever hemorrhage in infected embryos and it was similar in the two isolates, agglutination activity reflect titer (1024) for Najaf isolate and (512) for Baghdad isolate. The present study successfully detected part of (HN) gene by using specific primers designed for one-step RT-PCR undertaken to use them on molecular diagnosis of the hemagglutinin-neuraminidase (HN) gene in the Iraqi isolates and can be used for NDV virus detection in Iraq.