Aression of TLR9 in human pulmonary adenocarcinoma cell line A549

• Published in: The Chinese-German journal of clinical oncology Vol. 8; no. 7; pp. 393 - 396
• Main Authors: Yu, Jun, Pan, Tiecheng, Wei, Xiang, Liu, Ligang, Hu, Min, Yuan, Fang, Li, Jiaduo
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 2009
• Subjects: A549细胞; Cancer Research; Medicine; Medicine & Public Health; Oncology; Surgical Oncology; Toll样受体; 先天免疫; 实时荧光定量PCR; 支气管上皮细胞; 细胞株; 肺腺癌; 逆转录聚合酶链反应; HBE cell; A549 cell; immunocytochemistry; TLR; Real-time Quantitative PCR; western blot
• DOI: 10.1007/s10330-009-0069-8
• ISSN: 1610-1979

Objective： Being considered as a bridge between the innate immunity and acquired immunity, Toll-like receptors （TRLs） are very important innate immunity moleculars. Recent researchs on the innate immunity have focused on the relationship between TLRs and human tumor. This paper investgated the expression and significance of TLR9 in human pulmonary adenocarcinoma cell （A549 cell） and human bronchial epithelial cell （HBE cell）. Methods： After culturing A549 cell and HBE cell in vitro, the expression of TLR9 mRNA and protein in both cells were detected by immunocytochemistry, Real-time Quantitative Reverse Transcriptase-Polymerase Chain Reaction （Real-time Quantitative PCR） and Western blot, respectively. Results： By immunocytochemistry staining, TLR9 was mainly expressed in both cells＇ cell membrane and endochylema as brown-yellow material. It showed that the expressions of TLR9 mRNA and protein in A549 cell were stronger than those in H BE cell （P 〈 0.01）. Conclusion： The results suggest TLR9 might cause the progression of human pulmonary adenocarcinoma, and the mechanism needs to be further investgatied.