Aression of TLR9 in human pulmonary adenocarcinoma cell line A549
Objective: Being considered as a bridge between the innate immunity and acquired immunity, Toll-like receptors (TRLs) are very important innate immunity moleculars. Recent researchs on the innate immunity have focused on the relationship between TLRs and human tumor. This paper investgated the expre...
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Published in | The Chinese-German journal of clinical oncology Vol. 8; no. 7; pp. 393 - 396 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer-Verlag
2009
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Subjects | |
Online Access | Get full text |
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Summary: | Objective: Being considered as a bridge between the innate immunity and acquired immunity, Toll-like receptors (TRLs) are very important innate immunity moleculars. Recent researchs on the innate immunity have focused on the relationship between TLRs and human tumor. This paper investgated the expression and significance of TLR9 in human pulmonary adenocarcinoma cell (A549 cell) and human bronchial epithelial cell (HBE cell). Methods: After culturing A549 cell and HBE cell in vitro, the expression of TLR9 mRNA and protein in both cells were detected by immunocytochemistry, Real-time Quantitative Reverse Transcriptase-Polymerase Chain Reaction (Real-time Quantitative PCR) and Western blot, respectively. Results: By immunocytochemistry staining, TLR9 was mainly expressed in both cells' cell membrane and endochylema as brown-yellow material. It showed that the expressions of TLR9 mRNA and protein in A549 cell were stronger than those in H BE cell (P 〈 0.01). Conclusion: The results suggest TLR9 might cause the progression of human pulmonary adenocarcinoma, and the mechanism needs to be further investgatied. |
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Bibliography: | A549 cell Q753 Real-time Quantitative PCR Q969.436.5 TLR9; A549 cell; HBE cell; immunocytochemistry; Real-time Quantitative PCR; western blot TLR9 HBE cell immunocytochemistry western blot 42-1654/R |
ISSN: | 1610-1979 1613-9089 |
DOI: | 10.1007/s10330-009-0069-8 |