AG825 increases radiosensitivity of breast cancer cell

Objective: To observe the radiosensitivity effect of AG825 on breast cancer cell line with high expression of ERBB2 in vitro. Methods: MTT and clone formation assay were used to observe the effect of AG825 on proliferation and radiosensitivity of breast cancer line MDA-MB-453. After MDA-MB-453 was e...

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Bibliographic Details
Published inThe Chinese-German journal of clinical oncology Vol. 7; no. 10; pp. 587 - 589
Main Authors Luo, Bo, Yu, Shiying, Zhuang, Liang, Xia, Shu, Zhao, Zhen, Rong, Lei
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer-Verlag 2008
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Summary:Objective: To observe the radiosensitivity effect of AG825 on breast cancer cell line with high expression of ERBB2 in vitro. Methods: MTT and clone formation assay were used to observe the effect of AG825 on proliferation and radiosensitivity of breast cancer line MDA-MB-453. After MDA-MB-453 was exposed to AG825 and radiation, comet assay and Western blotting were applied to detect double strand break and expressions DNA-PKcs protein, respectively. Results: AG825 inhibited proliferation rate and decrease survival fraction of MDA-MB-453. After radiation, compared with control group, expression of DNA-PKcs was lower in group with AG825 presence but double strand break was higher. Conclusion: AG825 could increase radiosensitivity of breast cancer cell line MDA-MB-453, and it may associate with its inhibition of radia- tion induced expression of DNA-PKcs and double strand break repair.
Bibliography:AG825
R737.9
AG825; breast carcinomas; radiosensitivity
breast carcinomas
radiosensitivity
42-1654/R
ISSN:1610-1979
1613-9089
DOI:10.1007/s10330-008-0110-3