AG825 increases radiosensitivity of breast cancer cell

• Published in: The Chinese-German journal of clinical oncology Vol. 7; no. 10; pp. 587 - 589
• Main Authors: Luo, Bo, Yu, Shiying, Zhuang, Liang, Xia, Shu, Zhao, Zhen, Rong, Lei
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 2008
• Subjects: AG825; Cancer Research; Medicine; Medicine & Public Health; Oncology; Surgical Oncology; 乳腺癌细胞; 治疗方法; 辐射增敏作用; AG825; breast carcinomas; radiosensitivity
• ISSN: 1610-1979; 1613-9089
• DOI: 10.1007/s10330-008-0110-3

Objective： To observe the radiosensitivity effect of AG825 on breast cancer cell line with high expression of ERBB2 in vitro. Methods： MTT and clone formation assay were used to observe the effect of AG825 on proliferation and radiosensitivity of breast cancer line MDA-MB-453. After MDA-MB-453 was exposed to AG825 and radiation, comet assay and Western blotting were applied to detect double strand break and expressions DNA-PKcs protein, respectively. Results： AG825 inhibited proliferation rate and decrease survival fraction of MDA-MB-453. After radiation, compared with control group, expression of DNA-PKcs was lower in group with AG825 presence but double strand break was higher. Conclusion： AG825 could increase radiosensitivity of breast cancer cell line MDA-MB-453, and it may associate with its inhibition of radia- tion induced expression of DNA-PKcs and double strand break repair.