Effect of freezing on cord blood serum proteins

The effect of freezing regimes and storage temperatures on protein conformation and the spectrum of cord blood serum was investigated. Changes in the parameters of EPR spectra of spin probes in cord blood serum after slow freezing and subsequent thawing were established, indicating protein conformat...

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Published inBiophysics (Oxford) Vol. 54; no. 5; pp. 621 - 625
Main Authors Nardid, E. O., Rozanova, E. D., Tsymbal, L. V., Zinchenko, A. V., Nardid, O. A., Grishchenko, V. I.
Format Journal Article
LanguageEnglish
Published Dordrecht SP MAIK Nauka/Interperiodica 01.10.2009
Springer Nature B.V
Subjects
Biological and Medical Physics
Biophysics
Blood
Complex Systems Biophysics
Freezing
Physics
Physics and Astronomy
Proteins
microcalorimetry
spin probe EPR
serum
protein aggregation
gel chromatography
freezing regimes
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Summary:The effect of freezing regimes and storage temperatures on protein conformation and the spectrum of cord blood serum was investigated. Changes in the parameters of EPR spectra of spin probes in cord blood serum after slow freezing and subsequent thawing were established, indicating protein conformational changes characterized by loosening. This fact was confirmed by an earlier, first stage of albumin heat denaturation, as indicated by calorimetric data. It was shown that slow cooling resulted in aggregation of serum protein in which serum albumin and immunoglobulins played an important role. It was concluded that, for retaining the properties of cord blood serum proteins, it is preferable to perform cooling at a rate not lower than 100°C/min and a storage temperature of −80°C and lower.
ISSN:0006-3509
1555-6654
DOI:10.1134/S0006350909050121