Non-viral targeted delivery system mediates transfection of thymidine kinase gene of herpes simplex virus into ovarian cancer cells： a comparison between one time and continuous mediation

• Published in: The Chinese-German journal of clinical oncology Vol. 7; no. 6; pp. 368 - 371
• Main Authors: Liu, Xiaojun, Jin, Zhijun, Xie, Kangyun, Huo, Xiaoxi, Zhang, Xinyan, Liu, Yan, Xu, Congjian
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.06.2008
• Subjects: Cancer Research; I型单纯疱疹病毒; Medicine; Medicine & Public Health; Oncology; Surgical Oncology; 卵巢癌; 基因转导; 胸腺嘧啶核苷激酶; 靶向非病毒载体; ovarian cancer; gene therapy; targeted non-viral vector
• ISSN: 1610-1979; 1613-9089
• DOI: 10.1007/s10330-008-0069-0

Objective： To compare the transferring efficiency and killing effects of one time and continuous mediation with GE7, a non-viral targeted delivery system, in transfection of thymidine kinase gene of herpes simplex virus （HSV-tk） into ovarian cancer cells. Methods： GE7 was used to prepare recombinants with β-galactosidase （β-gal） and HSVI-tk; the recombinants were then used to transfect human ovarian cancer line CaOV3 once and continuously. β-gal staining was used to compare the efficiencies of one time and continuous mediation with GE7 system. Ganciclovior （GCV） was introduced into HSVI-tk transfected ovarian cells. Through drawing the cell growth curve and flow cytometry, the killing effects of GCV on once and continuously GE7/HSVI-tk transfected cells were observed. Results： We found that the one time and continuous exogenous gene transfer efficiencies were about 80% and 85%, respectively. When 1 μg/mL GCV was used to treat ovarian cell transfected with HSVI-tk gene, growth inhibiting rates of ovarian cells of one time and continuous transferring were 82% and 90%, respectively; their apoptosis indices were 15 and 30, respectively. Under same GCV concentration, continuous mediation of GE7/pCMV-tk transfection into ovarian cancer cells had more significant inhibitory effect than one time mediation （P 〈 0.05）. Conclusion： Compared with one time mediation, continuous mediation of transfection with GE7 gene delivery system has higher efficiency. Continuous mediation of GE7/HSVI-tk/GCV therapeutic gene system has more powerful killing effect.