Pyrene biodegradation by bacterial consortia enriched from coastal and marine sediments: Insights into bacterial community structures and functions

• Published in: Journal of environmental chemical engineering Vol. 12; no. 5; p. 113922
• Main Authors: Zeng, Huili, Huang, Jingyi, Wu, Minghuo, Hu, Minjie, Yang, Xiaojing, Zhou, Hao, Zhan, Jingjing, Zhang, Xuwang
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.10.2024
• Subjects: Bacterial consortia; Biodegradation; Coastal and marine sediment; Metabolic pathways; Pyrene; Biodegradation; Pyrene; Bacterial consortia; Coastal and marine sediment; Metabolic pathways
• ISSN: 2213-3437
• DOI: 10.1016/j.jece.2024.113922

As a representative polycyclic aromatic hydrocarbon (PAH), pyrene poses a significant threat to the environment and public health due to its persistence and potential toxicity. Extensive research has been conducted on biodegradation of pyrene, highlighting the crucial need to unravel its degradation mechanisms. Coastal and marine sediments are promising sources for discovering microbial candidates capable of degrading pyrene. In this study, three bacterial consortia, designated as BS, YS, and SS, were enriched from sediments collected from the Bohai Sea, Yellow Sea, and Liao River estuarine wetland, respectively. The bacterial consortia BS, YS, and SS demonstrated high pyrene degradation efficiencies, achieving 86.61 %, 80.39 %, and 75.70 % degradation at the concentration of 50 mg/L within 8 days, respectively. High-throughput sequencing of 16S rRNA gene amplicons revealed Pandoraea, Delftia, and Pseudomonas as the dominant populations in bacterial consortia BS, YS, and SS, respectively, with some species potentially being novel pyrene degraders. Further analysis included identifying pyrene degradation metabolites by LC-Q-TOF-MS (high-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry) and predicting functional genes through PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved Stats). Based on these results, the metabolic pathways for pyrene degradation by the bacterial consortia were proposed, which predominantly involved the dioxygenation pathway. Overall, these findings should offer valuable bacterial resources for facilitating the bioremediation of PAH-contaminated environments. •Bacterial consortia BS, YS, and SS could effectively degrade pyrene.•The metabolites of pyrene degradation by bacterial consortia were identified.•Pandoraea, Delftia and Pseudomonas were the dominant taxa for pyrene degradation.•Pyrene degradation pathways and the associated functional genes were proposed.