Monitoring of Erwinia amylovora in Montenegro
Recent studies of Erwinia amylovora in Montenegro, conducted from 2012 to 2014, indicated that the bacterium was widespread in the northern, continental part of the country, where the most important fruit-growing regions are situated. The presence of the bacterium was confirmed on quince, pear, appl...
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Published in | Pesticidi i Fitomedicina Vol. 30; no. 3; pp. 179 - 185 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Institute of Pesticides and Environmental Protection
01.09.2015
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Subjects | |
Online Access | Get full text |
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Summary: | Recent studies of Erwinia amylovora in Montenegro, conducted from 2012 to
2014, indicated that the bacterium was widespread in the northern,
continental part of the country, where the most important fruit-growing
regions are situated. The presence of the bacterium was confirmed on quince,
pear, apple, medlar and hawthorn. Pathogenic, cultural and biochemical
characteristics of E. amylovora strains sampled from pome fruit species and
indigenous flora in Montenegro had been studied previously. In the present
study, serological tests were used for identification of E. amylovora strains
originating from pome fruit trees and indigenous plants. Monitoring of E.
amylovora and collection of samples with symptoms of bacterial fire blight
from different hosts and locations were performed in Montenegro from 2012 to
2014. Isolation of the bacterium on nutrient medium produced a large number
of isolates, whose pathogenicity was confirmed on immature pear fruits.
Twenty-seven strains of the bacterium, originating from three pome fruit
species (quince, pear and apple) and one indigenous species (hawthorn) were
selected for serological analyses. Two applied serological methods, ELISA and
IF test, enabled rapid detection of the bacterium and simultaneous
examination of a large number of samples over a short period of time.
Serological analyses showed high homogeneity in antigenic structure of the
studied E. amylovora strains sampled from quince, pear, apple and hawthorn
from nine locations in Montenegro.
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ISSN: | 1820-3949 2406-1026 1820-3949 |
DOI: | 10.2298/PIF1503179R |